Transcription Termination: Pulling Out All the Stops

Mechanism of poly(A) signal transduction to RNA polymerase II in vitro.

Termination of transcription by RNA polymerase II usually requires the presence of a functional poly(A) site. How the poly(A) site signals its presence to the polymerase is unknown. All models assume that the signal is generated after the poly(A) site has been extruded from the polymerase, but this has never been tested experimentally. It is also widely accepted that a "pause" element in the DN...

Impact of hand forces and start/stop frequency on physiological responses to three forms of pushing and pulling: a South African perspective.

There has been limited attention given to the physiological demands of pushing and pulling, especially in industrially developing countries such as South Africa. Two key factors affecting the physiological demands of these tasks are the hand forces exerted and the start/stop frequency. The purpose of the current study was therefore to investigate the physiological responses to pushing and pulli...

Yarn pulling out test and numerical solution of penetration into woven fabric target impregnated with shear thickening fluid using SiO2 /Polyethylene Glycol

In this paper, finite element model of woven fabric target has been investigated which is impacted by a cylindrical projectile. Fabrics are impregnated with Shear Thickening Fluid (STF). The effects of the (STF) have been considered as frictional effect. The STF has been made (Nano Silica and Polyethylene Glycol (PEG)) and then diluted by ethanol proportion of 3:1. Yarn pulling out test from in...

New piezometer hammer and jack for easy installation and pulling out of the piezometer in drainage and ground water studies

Identification of a transcript release activity acting on ternary transcription complexes containing murine RNA polymerase I.

Termination of mammalian ribosomal gene transcription by RNA polymerase I (Pol I) requires binding of the nucleolar factor TTF-I (transcription termination factor for Pol I) to specific rDNA terminator elements. We have used recombinant murine TTF-I in an immobilized tailed template assay to analyze individual steps of the termination reaction. We demonstrate that, besides the TTF-I-DNA complex...